Don't you have any benchwork today?

Everyday, I go to lab and work my ass out, so that when I come back to my room, the only thing that I am able to do is plop on the bed and sleep. Now, once in a blue moon, I will be facing a mental block or am plainly having my period, so I don't feel like to doing any work. This is when you can easily catch me sitting and busily typing away in my laptop. I just want to left alone and without any disturbance. Now, I have a bunch of labmates who can be really caring at times. So, when they see me in front of my laptop, then the question that I most hate to hear comes out
" You don't have any benchwork today ?
I gritted my teeth, smiled and replied '' no-I dont have any benchwork today."
Seriously, my first thought was to tell her to mind her own work. I am in a very good mood to lash out at anyone at the slightest provocation today. But, it is simply not worth the energy so I decided to keep quiet. Her question sounded more like "Don't you have anything to do? or "Why are you so free today?'

This morning, I decided to put down that I was not in the mood today and am feeling lazy in Facebook. Well, that was to silent anyone who wanted to know why I am not doing my benchwork today. But, people don't usually understand that once it has been clearly stated that I am in no mood, I don't appreciate being bugged with questions like " Why no mood?"

Come on, just leave me alone. What? Is there any rules that I have to continuously work? Some other people just disappear and were never in the lab for hours, yet no one ask them anything..then. why bother me..this happens all the time.

Told you that I am in a good mood to write..

Angkor Watt- Cambodia

Recently, a friend went to Cambodia and visited Angkor Watt. I have always had a things for historic places. She brought back postcards and the photos were on Angkor Watt- it is a beautiful place. I was in fact thinking of visiting it -especially when the flight tickets are cheap.

Aphids and rice panicles

Aphids were attacking the rice panicles and I had to do something. Went to the storeroom and there were a few bottles of pesticides. One with permithryin as the active component listed out aphids as susceptibel to its activity. So, took it, diluted in water and sprayed on the plants. It has been raining continously and I was not in the mood to walk all the way to the green house. But, I can't see any aphids after a few hours the pesticide has been sprayed so may be it is working. It is really laborious to take care of the rice plants with the insetcs attacking and I think the plants are under orange leaf disease as well. The green house is in a bad condition thus is suceptible to all kinds of diseases. Hopefully, the panicles are able to bear seeds which will be used to generate the next stage of screening.

Politic is weird!

It has been a long time since I had updated this blog. Just got time to do it, with the server being down all the time. So, I had to spend some time in the computer room doing this. Today, in particular, I don't feel like doing any lab work. It is one of those days that you just want to be left on your own todo anything but lab work. I really have a few minor tasks to complete today and it won't take much time, but the mood is not there. The horoscope for today says -don't ignore your responsibilities no matter what-well, I am just not into it so here I am in fron of the computer, decided to write up in my blog. Was struggling to get it with the password and username not matching. It took me like 10 minutes and a few more minutes to fill up the -I FORGOT MY PASSWORD- form to realize that I have typed the username as .google.com and not as .gmail. com. Nothing's wrong with my password though and finally I got through. I am in a good mood to write a lot of stuffs, was a bit worried that I may have very little time to everything in my mind to words. I have been trashing myself for the pass two days to prepare MSO media for segregation analysis. The first attempt failed as the 6 bottles of media have not been autocalved well with the agar still visible at the bottom. It has not dissolved. So, I prepared another three and all should have turned out well except the fact that the macro was N6D and not MS-big mistke as the media did not solidify and stayed in liquid form- the color was yellowish instead of trasnparent. Dumped it into the decon bin and prepared again another three, this time with MS macro.checked the autocalve machine after two hours -only to discover that the bottles have not been autocalved. A power trip occured somewhere right after I had started the autocalve machine and it caused the machine to stop functioning. Luckily, the next person whom is just about to autoclave had agreed to autoclave my stuffs together. I was really frustated but kay since here I am, happily typing away and not doing any work at all.

Air-con broke down two weeks ago in the lab, the room is like a furnace. With the machines operating as usual, it is hot and sweaty in the room. I noticed that I feel tired easily in the room, sleepy and short -tempered. Anyhow, I will usually force myself to work, ignoring the heat- today, can't take it anymore. Not entering the room until it is 5 pm so that I can just pick my bag and leave.

Silly Mistakes can be really damaging

Hey there,

Today, is really a great day..(sigh..) I have been working on this particular clone for at least six months. Cloning it is really tough as the kit that we are using is not as efficient as claimed by the company. So, after all those while of cloning, transforming and analysing without any success, yesterday, out of four plates, I got a single colony. This is good enough as previously I was getting nothing at all. And there is one group in UM who are also working on a similar project-they got a single clone which contains the insert. So, i was hopeful that the hard work has ended. Instead, that evening itself I had mistakenly cultured the single colony with the entry clone antibiotic which is different from the destination vector's antibiotic. This is sickening and I JUST DISCOVERED IT THIS MORNING AFTER REMOVING IT FROM THE SHAKER. The culture did not turn cloudy, then it hit me that the antibiotic is wrong. I quickly went to the decon bin to serach for the plate that I HAVE DECIDED to throw away yesterday after culturing. All hopes of rescuing any of the clones streak was totally dampen with the fact that no matter what I can't find the plate. It is probably buried with the rest of the plates in the bin. I hate myself for this mistake. Now, I have to repeat the whole thing from the very start.

Comparing Blog entries

Have been busy surfing the net in the past few weeks and stumbled upon some politically inclined blogs..though it was fun to read through the comments, lashings and opinions..couldn't help noticing that the blogs were much better managed than what I call 'my blog'..Seriously, it's like this people spent a lot of time in front of their laptops/computers to micro manage their blogs..everything is written down to detal with references and replies to the comments..not to mention that these people are politicians or involved in politics in some way..no, I am not discriminating their ability..just shocked that the internet fever has caught on with everyone..initially, I was under the impression that politicians are being more approachable..you know, like answering questions from the public..with more tech savvy asking questions that probably you don't want to answer directly...the blog provides them a platform to think and device their answers..not to mention how certain blog entries are posted on initially before they were retracted after a few days when certain parties advice the blog admin to remove them..well, now the internet is no longer a safer place to put up your thoughts...

Mental stress due to delayed work

It has been a while since I last updated my blog. Today,my work has been delayed due to improper planning. Well, I did plan but plans were totally fucked up..so much for figuring it out all alone..it could haver been much better if I had talked it out with someone.

I seriuosly need to vent it out and funny the mood to write down something is much better when I am totally distracted with the fact that I can't do much of a work today..

Have been googling Prince William for quite some time..I never really fancied him but these few weeks, I have been having trouble getting him out of my system.Have been downloading a lot of his photos and updating news about him..

I am probably stressed out..stress has a weird way of taking a toll on me..feels much better to put all that is going on in my mind right now into words..I need to get a clear plan on how I should spent my time today productively..