Silly Mistakes can be really damaging

Hey there,

Today, is really a great day..(sigh..) I have been working on this particular clone for at least six months. Cloning it is really tough as the kit that we are using is not as efficient as claimed by the company. So, after all those while of cloning, transforming and analysing without any success, yesterday, out of four plates, I got a single colony. This is good enough as previously I was getting nothing at all. And there is one group in UM who are also working on a similar project-they got a single clone which contains the insert. So, i was hopeful that the hard work has ended. Instead, that evening itself I had mistakenly cultured the single colony with the entry clone antibiotic which is different from the destination vector's antibiotic. This is sickening and I JUST DISCOVERED IT THIS MORNING AFTER REMOVING IT FROM THE SHAKER. The culture did not turn cloudy, then it hit me that the antibiotic is wrong. I quickly went to the decon bin to serach for the plate that I HAVE DECIDED to throw away yesterday after culturing. All hopes of rescuing any of the clones streak was totally dampen with the fact that no matter what I can't find the plate. It is probably buried with the rest of the plates in the bin. I hate myself for this mistake. Now, I have to repeat the whole thing from the very start.

Comparing Blog entries

Have been busy surfing the net in the past few weeks and stumbled upon some politically inclined blogs..though it was fun to read through the comments, lashings and opinions..couldn't help noticing that the blogs were much better managed than what I call 'my blog'..Seriously, it's like this people spent a lot of time in front of their laptops/computers to micro manage their blogs..everything is written down to detal with references and replies to the comments..not to mention that these people are politicians or involved in politics in some way..no, I am not discriminating their ability..just shocked that the internet fever has caught on with everyone..initially, I was under the impression that politicians are being more approachable..you know, like answering questions from the public..with more tech savvy asking questions that probably you don't want to answer directly...the blog provides them a platform to think and device their answers..not to mention how certain blog entries are posted on initially before they were retracted after a few days when certain parties advice the blog admin to remove them..well, now the internet is no longer a safer place to put up your thoughts...

Mental stress due to delayed work

It has been a while since I last updated my blog. Today,my work has been delayed due to improper planning. Well, I did plan but plans were totally fucked up..so much for figuring it out all alone..it could haver been much better if I had talked it out with someone.

I seriuosly need to vent it out and funny the mood to write down something is much better when I am totally distracted with the fact that I can't do much of a work today..

Have been googling Prince William for quite some time..I never really fancied him but these few weeks, I have been having trouble getting him out of my system.Have been downloading a lot of his photos and updating news about him..

I am probably stressed out..stress has a weird way of taking a toll on me..feels much better to put all that is going on in my mind right now into words..I need to get a clear plan on how I should spent my time today productively..